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Image Search Results


Ground-based hardware used to simulate microgravity on Earth. A rotatory cell culture system (RCCS) equipped with HARVs and clinostat vessels rotated at different rotational axes: horizontal rotation to simulate microgravity (left) and vertical rotation for traditional gravity (right).

Journal: Microbiology Spectrum

Article Title: Microgravity effects on secondary metabolism of plant-affecting bacteria

doi: 10.1128/spectrum.02239-25

Figure Lengend Snippet: Ground-based hardware used to simulate microgravity on Earth. A rotatory cell culture system (RCCS) equipped with HARVs and clinostat vessels rotated at different rotational axes: horizontal rotation to simulate microgravity (left) and vertical rotation for traditional gravity (right).

Article Snippet: Both P. protegens and B. ambifaria were cultured using a clinostat ground-based microgravity simulation system (Synthecon).

Techniques: Cell Culture

Simulated microgravity affects the growth rate of P. protegens and B. ambifaria . ( A ) Growth curve of ( A ) P. protegens and ( B ) B. ambifaria under simulated microgravity (SMG), traditional gravity (TG), and orbital shaking (OS) control. X-axis values represent hours.

Journal: Microbiology Spectrum

Article Title: Microgravity effects on secondary metabolism of plant-affecting bacteria

doi: 10.1128/spectrum.02239-25

Figure Lengend Snippet: Simulated microgravity affects the growth rate of P. protegens and B. ambifaria . ( A ) Growth curve of ( A ) P. protegens and ( B ) B. ambifaria under simulated microgravity (SMG), traditional gravity (TG), and orbital shaking (OS) control. X-axis values represent hours.

Article Snippet: Both P. protegens and B. ambifaria were cultured using a clinostat ground-based microgravity simulation system (Synthecon).

Techniques: Control

Relative production of select plant-promoting secondary metabolites under traditional gravity and simulated microgravity in P. protegens and B. ambifaria . ( A ) Pyoluteorin levels, ( B ) 2,4-DAPG levels, and ( C ) orfamide A levels on Day 1 and Day 3 in P. protegens cultured in TG and SMG conditions. ( D ) PQQ levels in B. ambifaria cultured in TG and SMG conditions on Day 1 and Day 3. Metabolite levels were measured via LC-MS/MS and are shown as maximum intensity values ( n = 3 biological replicates per condition). Bars show mean ± SD of three biological replicates ( n = 3), normalized to OD₆₀₀. Statistical significance was determined using a two-tailed Welch’s t -test; P < 0.05 (*), P < 0.01 (**), ns = not significant.

Journal: Microbiology Spectrum

Article Title: Microgravity effects on secondary metabolism of plant-affecting bacteria

doi: 10.1128/spectrum.02239-25

Figure Lengend Snippet: Relative production of select plant-promoting secondary metabolites under traditional gravity and simulated microgravity in P. protegens and B. ambifaria . ( A ) Pyoluteorin levels, ( B ) 2,4-DAPG levels, and ( C ) orfamide A levels on Day 1 and Day 3 in P. protegens cultured in TG and SMG conditions. ( D ) PQQ levels in B. ambifaria cultured in TG and SMG conditions on Day 1 and Day 3. Metabolite levels were measured via LC-MS/MS and are shown as maximum intensity values ( n = 3 biological replicates per condition). Bars show mean ± SD of three biological replicates ( n = 3), normalized to OD₆₀₀. Statistical significance was determined using a two-tailed Welch’s t -test; P < 0.05 (*), P < 0.01 (**), ns = not significant.

Article Snippet: Both P. protegens and B. ambifaria were cultured using a clinostat ground-based microgravity simulation system (Synthecon).

Techniques: Cell Culture, Liquid Chromatography with Mass Spectroscopy, Two Tailed Test